Membrane protein gRNA library

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CRISPR-iScreen™ Library Plasmid

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

Membrane protein gRNA library

Catalog#： LIBR-H006-P002 LIBR-H006-P100 LIBR-H006-P200 LIBR-H006-P500

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Detailed Product Information Recommended Companion Products

The CRISPR activation library targets 6,213 human membrane protein genes with a total of 58,570 gene activation plasmid vectors, of which 9 different gRNA vectors designed for each gene, in addition to 500 control vectors targeting intergenic sequences. The library uses pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W as the backbone, which is a triple plasmid system that expresses only gRNA, while the dCas9-VP64 and MS2-P65-HSF1 genes are on two other different vectors that need to be used in combination.

Product Name

Membrane protein gRNA library

Species

Human

Library Type

Activation Library

Plasmid System

Triple-plasmid System

Virus Packaging System

3rd Lentivirus Packaging System

Targeted Genes

6213

gRNA Number

58570

Non-targeting gRNA Number

500

Selection Marker

Puro, TagBFP

Backbone Map

pKVL2-U6gRNA_SAM(BbsI)-PGKpuroBFP-W vector formula

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CRISPR iScreen™ Product Strength

35+ Libraries
100+ Cas9 cell lines for screening
35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
1
Plasmid
Coverage>99%, uniformity<10
The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
2
Cell Pool
Coverage rate up to 99%
Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
3

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Membrane protein gRNA library

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Membrane protein gRNA library

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